Thermo Fisher Scientific S-peptide Epitope Tag Polyclonal Antibody
S-peptide 태그를 인식하는 Thermo Fisher의 Rabbit Polyclonal Antibody로 다양한 태그 단백질 검출에 사용. WB, ICC/IF, IP 등 다중 응용 가능. 보존제 무첨가로 고순도. 단기 4°C, 장기 -20°C 보관 권장.
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Thermo Fisher Scientific S-peptide Epitope Tag Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunocytochemistry (ICC/IF) | 1:300–1:10,000 |
| Immunoprecipitation (IP) | 0.5–2 µg/mg of lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to the S-tag sequence (KETAAAKFERQHMDS) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A, Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2788824 |
Product Specific Information
- This product is preservative-free.
- It is recommended to add sodium azide (final concentration 0.05%–0.1%) to avoid contamination.
- This antibody recognizes both N-terminal and C-terminal S tags in fusion proteins.
Target Information
The S-Peptide epitope tag (S-Tag™) is used for analysis of localization, expression detection, and purification of polypeptides from mammalian cells.
Its small size (15 amino acids), ability to purify tagged polypeptides without antibodies, and minimal endogenous reactivity make it an excellent alternative to other epitope tags.
Epitope tagging enables detection and purification of proteins expressed by cloned genes. Expression vectors are engineered to include a nucleotide sequence encoding the peptide tag, allowing fusion protein synthesis. Detection and purification are mediated by antibodies specific to the engineered peptide.
Notes
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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