Thermo Fisher Scientific CD34 Monoclonal Antibody (MEC14.7)
CD34 단백질을 인식하는 Rat 모노클로날 항체(MEC14.7)로, 줄기세포 표지 연구에 적합. WB, IHC, Flow Cytometry 등 다양한 응용 가능. Unconjugated 형태로 제공되며, Biotin, FITC, PE 포맷도 선택 가능. 단기 4°C, 장기 -20°C 보관.
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Thermo Fisher Scientific CD34 Monoclonal Antibody (MEC14.7)
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | Assay-dependent | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent | 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent | - |
| Flow Cytometry (Flow) | 0.5 µg/1x10^6 cells | 3 publications |
| ELISA | Assay-dependent | - |
| Immunoprecipitation (IP) | Assay-dependent | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MEC14.7 |
| Immunogen | Murine transformed endothelioma cell line t-end |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C (avoid freeze/thaw cycles) |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2539209 |
Available Conjugate Formats
Target Information
CD34 is a highly glycosylated monomeric surface protein (111–115 kDa) expressed on various stem cell populations. It functions as a stem cell marker and may act as a surface receptor involved in receptor-mediated endocytosis, adhesion, differentiation, and proliferation of hematopoietic stem cells and progenitors. CD34 expression indicates specific stages of hematopoietic development and may mediate attachment to bone marrow extracellular matrix or stromal cells. It also serves as a scaffold for lineage-specific glycans and may present carbohydrate ligands to selectins. The intracellular domain can be phosphorylated by activated protein kinase C, suggesting a role in signal transduction. CD34 dysfunction is associated with dermatofibrosarcoma and neurofibroma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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