Thermo Fisher Scientific UBCH7 Polyclonal Antibody
Thermo Fisher Scientific의 UBCH7 Polyclonal Antibody는 인간 UBE2L3 단백질의 100–154번 잔기 영역을 면역원으로 제작된 Rabbit IgG 항체입니다. Western blot, IHC, IP 등 다양한 응용에 적합하며, 액상형으로 4°C에서 보관합니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:1,000–1:5,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000–1:5,000 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Property | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 100 and the C-terminus (residue 154) of Human Ubiquitin-Conjugating Enzyme H7 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections
- Based on 100% sequence identity, this antibody is predicted to react with Bovine and Orangutan
Target Information
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation.
Ubiquitination involves at least three classes of enzymes:
- Ubiquitin-activating enzymes (E1s)
- Ubiquitin-conjugating enzymes (E2s)
- Ubiquitin-protein ligases (E3s)
UBE2L3 is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme participates in the ubiquitination of p53, c-Fos, and the NF-κB precursor p105 in vitro.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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