Thermo Fisher Scientific CCT4 Monoclonal Antibody (OTI4C9), TrueMAB
CCT4 단백질을 인식하는 마우스 모노클로날 항체로 Western blot 및 IHC(파라핀 절편)에 적합. 인간, 마우스, 랫트 반응성. 동결건조 형태로 제공되며, 재구성 후 약 1 mg/mL 농도. 연구용으로 단백질 접힘 관련 연구에 활용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI4C9 |
| Immunogen | Full length human recombinant protein of human CCT4 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
The chaperonin containing TCP1 (CCT) complex, also known as the TCP1 ring complex, consists of two back-to-back rings, each containing eight unique but homologous subunits, including CCT4.
CCT assists in folding newly translated polypeptides through ATP-driven cycles of release and rebinding of partially folded intermediates.
Known substrates include cytoskeletal proteins actin and tubulin, as well as alpha-transducin (Won et al., 1998 [PubMed 9819444]).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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