Invitrogen™

TOPO™ XL-2 Complete PCR Cloning Kit, without competent cells

The TOPO™ XL-2 Complete PCR Cloning Kit provides all the necessary elements for efficient cloning of extra-long PCR products (up자세히 알아보기

The TOPO™ XL-2 Complete PCR Cloning Kit provides all the necessary elements for efficient cloning of extra-long PCR products (up to 13 kb). The kit uses the linearized and topoisomerase 1-activated pCR™-XL-2-TOPO™ vector, which is compatible with the cloning of blunt-end PCR fragments. Amplification of long PCR fragments is enabled by Platinum™ SuperFi™ Green PCR Master Mix, which is included in the kit. Topoisomerase I activation of the vector allows PCR products to be ligated in just 5 minutes on your bench–top, resulting in high cloning efficiency (up to 90% positive recombinants with 10 kb fragments).

The pCR-XL-2-TOPO vector includes:
• ccdB gene for positive selection
• EcoR I site flanking the PCR product insertion site for easy excision of inserts
• Ampicillin- and kanamycin-resistance genes for your choice of selection in E. coli
• T7 promoter/priming site for in vitro RNA transcription and sequencing
• T7, T3, and M13 forward and reverse primer sites for sequencing

The TOPO XL-2 Complete PCR Cloning Kit includes:
• TOPO XL-2 PCR Cloning Kits containing the pCR-XL-2-TOPO vector
• Platinum SuperFi Green PCR Master Mix—featuring a proofreading DNA polymerase (>100X fidelity compared to Taq) with high processivity and a density gradient to generate accurate, long PCR amplicons ready to load onto an agarose gel for gel extraction
• PureLink™ Quick Gel Extraction and PCR Purification Combo Kit—designed to purify DNA fragments from agarose gels or direct PCR purification using a silica-based spin cartridge in < 30 minutes

For a kit that includes competent cells, please see Cat. Nos. K805010 and K805020.

사양

박테리아 또는 효모 균주Not Included

항생제 내성 박테리아Ampicillin (AmpR), Kanamycin (KanR)

클로닝 방법Zero Blunt TOPO

형식Kit

용도(애플리케이션)PCR Cloning

반응 수10 Reactions

제품라인TOPO™

제품 유형Cloning Kit

프로모터T3, T7

수량10 reactions

배송 조건Approved for shipment at Room Temperature or on Wet or Dry Ice

벡터pCR-XL-2-TOPO

Unit SizeEach