Thermo Fisher Scientific F(ab`)2-Goat anti-Human IgG (H+L) Secondary Antibody, HRP
HRP 결합 F(ab')2 형태의 염소 유래 항-인간 IgG(H+L) 2차 항체로, WB, IHC, ELISA 등에 사용 가능. 고순도의 친화 크로마토그래피 정제 제품이며, 높은 특이성과 감도를 제공. 동결건조 형태로 제공되어 안정적 보관 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:20,000 |
| Immunohistochemistry (IHC) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 2.5 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.1% ProClin 150 |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2535933 |
| Target | IgG |
| Antibody Form | F(ab`)2 Fragment |
Available Conjugate Formats
- Alexa Fluor 488
- Alexa Fluor 647
- Alexa Fluor Plus 488
- Alexa Fluor Plus 647
- AP
- Biotin
- FITC
- PE
- Qdot 655
- Rhodamine
- Texas Red
- TRITC
- Custom conjugation available upon request
Product Specific Information
- Each lot’s sensitivity is verified by ELISA and specificity confirmed by immunoelectrophoresis (IEP).
- Rehydrate with 0.55 mL deionized water; allow 30 minutes at room temperature to dissolve.
- Centrifuge to remove particulates and prepare fresh working dilution daily.
- Store lyophilized material at 2–8 °C.
- For long-term storage after reconstitution, dilute with 50% glycerol and store at −20 °C.
- Based on immunoelectrophoresis, no reactivity is observed to non-immunoglobulin human serum components.
Target Information
Anti-Human secondary antibodies are affinity-purified with well-characterized specificity for human immunoglobulins. They are useful for detection, sorting, or purification of target proteins. Secondary antibodies enhance versatility and sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody. Typically produced by immunizing host animals with pooled immunoglobulins from the target species, then purified and modified (e.g., immunoaffinity chromatography, fragmentation, or conjugation) for high specificity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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