Thermo Fisher Scientific NCE2 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.05-0.2 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

2-4 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide sequence (HLRDKEDFRNKVDD) corresponding to the C-terminus amino acids of UBE2F (aa 165-178). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:NCE2,uniProtId:Q969M7-1,ncbiNodeId:9606,antigenRange:165-178,antigenLength:185,antigenImageFileName:PA5-19029_NCE2_Q969M7-1_Goat.svg,antigenImageFileNamePDP:PA5-19029_NCE2_Q969M7-1_Goat_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Ammonium sulfate precipitation

Storage buffer

TBS, pH 7.3, with 0.5% BSA

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_10974332

Product Specific Information

This antibody is predicted to react with bovine, canine and rat based on sequence homology.

This antibody is tested in Peptide ELISA: antibody detection limit dilution 32,000.

Target Information

Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.