Thermo Fisher Scientific Cytokeratin pan (Epithelial Marker) Recombinant Superclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

2-4 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

0.25-0.5 µg/mL

-

Immunocytochemistry (ICC/IF)

1-3 µg/mL

-

Flow Cytometry (Flow)

1-2 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG, kappa

Expression System

HEK293 cells

Class

Recombinant Superclonal

Type

Antibody

Immunogen

Recombinant full-length human KRT76 and KRT77 proteins if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Cytokeratin Pan Type I/II,uniProtId:Q01546-1,ncbiNodeId:9606,antigenRange:1-638,antigenLength:638,antigenImageFileName:RBP35-1835-P1NEO_Cytokeratin_Pan_Type_III_Q01546-1_Rabbit.svg,antigenImageFileNamePDP:RBP35-1835-P1NEO_Cytokeratin_Pan_Type_III_Q01546-1_Rabbit_PDP.jpeg,sortOrder:17},{targetFamily:Cytokeratin Pan Type I/II,uniProtId:Q7Z794-1,ncbiNodeId:9606,antigenRange:1-578,antigenLength:578,antigenImageFileName:RBP35-1835-P1NEO_Cytokeratin_Pan_Type_III_Q7Z794-1_Rabbit.svg,antigenImageFileNamePDP:RBP35-1835-P1NEO_Cytokeratin_Pan_Type_III_Q7Z794-1_Rabbit_PDP.jpeg,sortOrder:18}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

200 µg/mL

Purification

Protein A

Storage buffer

PBS with 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Antibody is stable for 24 months.

Positive Control: Skin, Adeno- or Squamous carcinomas Cellular Localization: Cytoplasmic

Specificity Comments: Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, which 67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8); 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19). Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. It is a broad spectrum anti pan-cytokeratin antibody, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adeno carcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It may be useful to characterize the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and has high sensitivity in the recognition of epithelial cells and carcinomas.

Shows broad species reactivity

Target Information

Cytokeratin pan is part of a subfamily of intermediate filament proteins that are characterized by remarkable biochemical diversity, and represented in human epithelial tissues by at least 20 different polypeptides. Cytokeratins range in molecular weight between 40 kDa- 68 kDa, and an isoelectric pH between 4.9-7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays. The composition of cytokeratin pairs vary with the epithelial cell type, stage of differentiation, cellular growth environment, and disease state. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.