Thermo Fisher Scientific CXCL9 (MIG) Monoclonal Antibody (MIG-2F5.5), PE, eBioscience

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.25 µg/test

View 2 publications 2 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Armenian hamster / IgG

Recommended Isotype Control

Armenian Hamster IgG Isotype Control (eBio299Arm), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

MIG-2F5.5

Conjugate

PE PE PE

View additional formats

• eFluor 660
• Request custom conjugation

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_891582

Product Specific Information

Description: The monoclonal antibody MIG-2F5.5 reacts with mouse monokine induced by IFN gamma (mig, mig-1, CXCL9). This 14.4 kDa inflammatory chemokine is specifically induced by IFN gamma, but not other activators such as LPS or IFN alpha. Secretion of mig, mainly by macrophages results in the chemotaxis of a variety of activated T cells via the CXCR3 chemokine receptor. Mig is involved many areas of research including autoimmune diseases, cancer, and inflammation.

Applications Reported: This MIG-2F5.5 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This MIG-2F5.5 antibody has been tested by intracellular staining followed by flow cytometric analysis of IFN gamma-stimulated thioglycolate-elicited peritoneal exudate cells and RAW 264.7 cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

MIG, also known as CXCL9, is a T-cell chemoattractant inducible by gamma interferon that is a member of the CXC chemokine family of cytokines.This gene is mapped to 4q21. It is noted that, although the best-described activities of the chemokines are as chemotactic factors, chemokines also have an effect on T-cell activation, angiogenesis, and HIV infection. While most CXC chemokines are chemotactic for neutrophils, MIG and INP10 are unusual and similar in being CXC chemokines that are chemotactic for lymphocytes and inactive in neutrophils.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.