
Thermo Fisher Scientific CXCL9 (MIG) Monoclonal Antibody (MIG-2F5.5), PE, eBioscience
Mouse CXCL9(MIG) 단백질을 인식하는 Armenian hamster 유래 단일클론 항체로, PE 형광 표지되어 있습니다. Flow cytometry용으로 검증되었으며, IFN-γ 자극 세포 내 염색에 적합합니다. 0.2 mg/mL 농도, PBS buffer, 4°C 암저장.
- 카탈로그번호
- 12-3009-80
- 판매단위
- pk
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1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CXCL9 (MIG) Monoclonal Antibody (MIG-2F5.5), PE, eBioscience
Thermo Fisher Scientific CXCL9 (MIG) Monoclonal Antibody (MIG-2F5.5), PE, eBioscience
Applications
- Flow Cytometry (Intracellular staining)
Tested Dilution
- ≤ 0.25 µg/test
Publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Armenian hamster / IgG |
| Recommended Isotype Control | Armenian Hamster IgG Isotype Control (eBio299Arm), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MIG-2F5.5 |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_891582 |
Product Specific Information
The monoclonal antibody MIG-2F5.5 reacts with mouse monokine induced by IFN gamma (MIG, MIG-1, CXCL9). This 14.4 kDa inflammatory chemokine is specifically induced by IFN gamma, but not by LPS or IFN alpha. MIG secretion, mainly by macrophages, results in chemotaxis of activated T cells via the CXCR3 receptor. MIG is involved in autoimmune disease, cancer, and inflammation research.
Applications Reported:
This antibody is suitable for intracellular staining followed by flow cytometric analysis.
Applications Tested:
Tested by intracellular staining of IFN-γ stimulated thioglycolate-elicited peritoneal exudate cells and RAW 264.7 cells. Use ≤ 0.25 µg per test (100 µL final volume). Optimal titration recommended.
Excitation / Emission / Laser:
- Excitation: 488–561 nm
- Emission: 578 nm
- Laser: Blue, Green, Yellow-Green
Filtration:
0.2 µm post-manufacturing filtered.
Target Information
MIG (CXCL9) is a T-cell chemoattractant induced by gamma interferon, belonging to the CXC chemokine family. Mapped to 4q21, it influences T-cell activation, angiogenesis, and HIV infection. Unlike most CXC chemokines that attract neutrophils, MIG and INP10 are chemotactic for lymphocytes and inactive in neutrophils.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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