Thermo Fisher Scientific CDH11 Monoclonal Antibody (5B2H5)
Thermo Fisher Scientific의 CDH11 Monoclonal Antibody (5B2H5)는 인간 및 마우스 Cadherin 11 단백질에 특이적으로 반응하는 항체입니다. Western blot, IHC, IP 등 다양한 응용에 적합하며, 교차 반응이 없는 고특이성 항체입니다. 단백질 A로 정제된 액상 형태로 제공됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 2–4 µg/mL | View 24 publications |
| Immunohistochemistry (IHC) | 5–10 µg/mL | View 12 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | – | View 2 publications |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | – | View 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | – | View 1 publication |
| Immunocytochemistry (ICC/IF) | – | View 7 publications |
| Flow Cytometry (Flow) | – | View 1 publication |
| Immunoprecipitation (IP) | 5 µg | View 1 publication |
| ChIP assay (ChIP) | – | View 1 publication |
| in situ PLA (PLA) | – | View 1 publication |
| Miscellaneous PubMed (Misc) | – | View 7 publications |
Product Specifications
| Specification | Details |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Bovine, Chicken, Dog, Human, Mouse, Pig, Rat |
| Host/Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5B2H5 |
| Immunogen | Recombinant protein derived from an intracellular sequence of the human Cadherin 11 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.1% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2533068 |
Product Specific Information
This antibody is specific for the ~110 kDa Cadherin 11 protein. Cross-reactivity with other Cadherin family members has not been observed. It is confirmed reactive with human and mouse Cadherin 11. Reactivity was validated on western blots using MDA.MB231 cells (human) and mouse L fibroblast lysates.
For immunohistochemistry of frozen sections, incubate for 30–60 minutes at room temperature or overnight at 4°C.
Target Information
The CDH11 gene encodes a type II classical cadherin, part of the cadherin superfamily of calcium-dependent cell-cell adhesion molecules. Classical cadherins contain five N-terminal extracellular cadherin (EC) domains, a transmembrane domain, and a conserved cytoplasmic domain.
Type II cadherins, including cadherin-5, -6, -8, -11, and -12, lack the HAV cell adhesion recognition sequence found in type I cadherins.
Cadherin-11 (OB-Cadherin) serves as a marker for mesenchymal, loosely connected, and migratory cells. It is strongly expressed in brain, spinal cord, bone marrow, and bone cells, and plays a role in bone development and maintenance.
A variant form of Cadherin-11, generated by alternative splicing, encodes a truncated protein with an unusual cytoplasmic domain. Cadherin-11 expression is associated with invasive breast cancer cell lines, suggesting its role as a marker for aggressive tumor subsets.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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