Thermo Fisher Scientific HisPur Cobalt Purification Kit, 0.2 mL

Thermo Scientific™ HisPur™ Cobalt Purification Kit, 0.2 mL

The Thermo Scientific HisPur Cobalt Purification Kit contains 0.2 mL HisPur spin columns, buffers, and collection tubes for purification of polyhistidine-tagged proteins. The HisPur Cobalt Resin in these columns is a tetradentate chelating agarose resin charged with divalent cobalt (Co²⁺), enabling high-purity His-tagged protein purification with minimal metal contamination.

Features of HisPur Cobalt Resin

• High purity — Obtain more than 10 mg of pure His-tagged protein per mL of resin without optimizing imidazole washing conditions
• Specificity — Cobalt-chelate coordination binds fewer host protein contaminants, yielding lower background than nickel resins
• Low metal leaching — No metal contamination in eluted samples
• Versatility — Suitable for native or denaturing conditions; compatible with Thermo Scientific Pierce Cell Lysis Reagents and various buffer additives
• Cost effective — Resin can be reused or discarded after single use
• Flexibility — Available as bulk resin, kits, predispensed columns, chromatography cartridges, and 96-well filter plates

Performance Comparison

Compared to Ni-IDA and tetradentate Ni²⁺ chelate resins, HisPur Cobalt Resin provides higher specificity and gentler elution (lower imidazole concentration). While nickel resins can yield high protein amounts, they often require additional cleanup due to nonspecific binding. HisPur Cobalt Resin minimizes nonspecific interactions, metal leaching, and allows for milder elution conditions, maximizing protein purity without sacrificing yield.

Binding Capacity

HisPur Cobalt IMAC Resin specifically binds His-tagged recombinant proteins that can be efficiently recovered by mild imidazole elution. More than 10 mg of His-tagged protein can be bound per mL of resin using a 28 kDa fusion protein expressed and purified from an E. coli lysate. Various His-tagged proteins can be recovered in similar or higher purity compared to other nickel or cobalt IMAC resins using spin or gravity-flow formats.

Available Formats

• Resin Slurries: Crosslinked 6% beaded agarose; 10 mL, 100 mL, 500 mL bottles
• Spin Columns: 0.2 mL (microcentrifuge), 1 mL, and 3 mL columns
• Purification Kits: Complete kits in all three column sizes
• Chromatography Cartridges: Learn more and see HisPur Chromatography Cartridge data
• 96-well Spin Plates

Related Products

• HisPur™ Cobalt Resin
• HisPur™ Cobalt Spin Columns, 0.2 mL

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