Invitrogen™

One Shot™ MAX Efficiency™ DH5α-T1R Competent Cells

One Shot MAX Efficiency DH5α-T1R Competent Cells are derived from the popular DH5α strain and carry the tonA (fhuA) mutation자세히 알아보기

One Shot MAX Efficiency DH5α-T1R Competent Cells are derived from the popular DH5α strain and carry the ton_A (f_huA) mutation that confers resistance to T1 and T5 phage. They offer added security to protect valuable clones and libraries. This is especially important for genome and sequencing centers where T1 phage infection would be detrimental.

DH5α-T1R is a versatile strain that can be used in many everyday cloning applications. Prepared by a proprietary modification of the Hanahan method, these cells are suitable for the construction of gene banks or for the generation of cDNA libraries using plasmid-derived vectors. The _lacZ_∆M15 marker provides α-complementation of the ß-galactosidase gene from pUC or similar vectors and therefore can be used for blue/white screening of colonies on bacterial plates containing Bluo-gal or X-gal. DH5α-T1R Competent Cells are capable of being transformed efficiently with large plasmids and can also serve as a host for the M13mp cloning vectors if a lawn of DH5αF`IQ is provided to allow plaque formation.

Benefits of MAX Efficiency DH5α-T1R Competent Cells
• Transformation efficiency of >1 x 109 transformants/μg with non-saturating amounts of DNA
• ton_A (_fhuA) mutation to confer resistance to T1 and T5 phage
• end_A1 mutation that enables cleaner DNA preparations and better results in downstream applications due to the elimination of nonspecific digestion by Endonuclease I
• _rec_A1 mutation that reduces occurrence of unwanted recombination in cloned DNA
• _hsdR for efficient transformation of unmethylated DNA from PCR applications
• _lac_ZΔM15 marker that allows blue/white color screening of recombinant clones carrying the alpha fragment of β-galactosidase

Convenient and efficient format
DH5α-T1R E. coli are supplied in the convenient, single-reaction One Shot format. Each tube contains enough cells for one transformation so there are no efficiency-zapping, freeze-thaw cycles or money wasted on unused cells.

Genotype: F- φ80_lac_ZΔM15 Δ(lacZYA-_arg_F)U169 _rec_A1 _end_A1 _hsd_R17(rK-, mK+) _pho_A _sup_E44 _thi_-1 _gyr_A96 _rel_A1 _ton_A

Find the strain and format that fits you needs
DH strains are available in chemically competent and electrocompetent cell formats.
MAX Efficiency DH5αF´IQ Competent Cells are available in chemically competent format.
DH5α-T1R is available in Multishot format for high throughput applications.

사양

제품 유형Chemically Competent Cells

에프에피솜 포함No

플라스미드 품질 개선Yes (endA1)

메틸화 DNA 클로닝No

형질전환 효율 수준High Efficiency (>1 x 109 cfu/μg)

항생제 내성 박테리아No

불안정 DNA 클로닝Not suitable for cloning unstable DNA

블루/화이트 스크리닝Yes (lacZΔM15)

고처리량 호환성Low

비메틸화 DNA 준비No

재조합 감소Yes (recA1)

배송 조건Dry Ice

T1 Phage - 저항성(tonA)Yes

종E. coli (K12)

형식Tube

제품라인One Shot™

수량21 x 50 μL

Unit SizeEach