Thermo Fisher Scientific Rat anti-Mouse IgG2b Secondary Antibody, FITC

Applications

Tested Dilution

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

ELISA (ELISA)

Assay-dependent

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Rat / IgG, kappa

Class

Monoclonal

Type

Secondary Antibody

Clone

LO-MG2b-2

Immunogen

Immunoglobulins.

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Purification

purified

Storage buffer

PBS

Contains

0.1% sodium azide

Storage conditions

Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C. Store in the dark.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1076013

Target

IgG2b

Antibody Form

Whole Antibody

Product Specific Information

Mouse Gamma 2b Heavy Chain of Immunoglobulin (determined by immunodot). positively tested on BALB/c and C57BL/6 mouse sera. The specificity of every rat monoclonal antibody anti-mouse IgG subclasses is determined in a range of optimal concentrations. Increasing the first or the second antibody at an unnecessary too high concentration can induce possible cross-reactions with other mouse IgG subclasses. Avidity on IgG2b: 1 * 1010 M-1. No cross-reactivity with chicken, rabbit, goat, sheep, bovine, horse, dog, swine, baboon IgG and human IgG or IgM (ELISA test). Available unconjugated MON5055, FITC MON5055F, Biotin MON5055B, PE MON5055P

Target Information

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.