Thermo Fisher Scientific Human IL-2 Recombinant Protein, eBioscience

Applications

Tested Dilution

Publications

Control (Ctrl)

Assay-Dependent

-

Product Specifications

Species

Human

Expression System

E. coli

Amino acid sequence

Human IL-2 recombinant protein contains 133 amino acids. It contains and N-terminal Met and a A126C substitution.

Molecular weight

15.4 kDa

Class

Recombinant

Type

Protein

Purity

>99%

Activity

ED50 < 0.0645 ng/mL; determined by dose-dependent stimulation of murine CTLL-2 cells. This corresponds to a specific activity of 1.55x10e7 IU/mg.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Wet ice

Product Specific Information

Description: Recombinant human IL-2 produced in E.Coli is a single, non-glycosylated mutein (variant form) of human IL-2 polypeptide chain containing 133 amino acids and having a molecular mass of 15385.79 Dalton. eBioscience`s rhIL-2 is Des N-Terminal Met and has an Ala substitute for Cysteine at position 126.

BMS334 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of murine CTLL-2 cells is < 0.0645 ng/mL, corresponding to a specific activity of 1.55x107 IU/mg.

Purity: >99%.

Molecular Weight: 15.4 kDa.

Target Information

Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.