Thermo Fisher Scientific ALAS1 Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 0.2–1 µg/mL

Product Specifications

Product Specific Information

Immunogen sequence:
ETSAGPSVVS VKTDGGDPSG LLKNFQDIMQ KQRPERVSHL LQDNLPKSVS

• Short-term storage: 2–8°C up to 1 week
• Long-term storage: -20°C in small aliquots to prevent freeze-thaw cycles

Predicted homology:
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 93%; Rat: 100%; Sheep: 93%; Zebrafish: 86%

Target Information

ALAS-1 is a nuclear-encoded mitochondrial matrix enzyme that catalyzes the condensation of glycine with succinyl-CoA to form delta-aminolevulinate, CO₂, and CoA. It regulates the first and rate-limiting step of the heme biosynthetic pathway.
This pyridoxal-5'-phosphate-dependent housekeeping enzyme is ubiquitously expressed and provides heme for cytochromes and other hemoproteins.

In the liver, ALAS1 undergoes negative feedback regulation by heme. Its transcription is stimulated by cAMP and respiratory uncoupling, while phorbol esters and insulin repress its expression.
ALAS1 is implicated in the reciprocal regulation of heme biosynthesis and the circadian clock, suggesting its potential as a therapeutic target for circadian disorders.
Downregulation of ALAS1 is observed in acute liver failure, leading to altered heme metabolism and liver dysfunction.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.