Invitrogen™

EnzChek™ Lysozyme Assay Kit

The EnzChek™ Lysozyme Assay Kit provides researchers with a simple and sensitive assay to measure lysozyme activity in solution. The자세히 알아보기

The EnzChek™ Lysozyme Assay Kit provides researchers with a simple and sensitive assay to measure lysozyme activity in solution. The increase in fluorescence resulting from lysozyme activity is measured using a fluorometer or fluorescence microplate reader.

See our complete line of Fluorescence Microplate assays.

• Detect lysozyme activity concentrations as low as 20 U/mL
• Use standard fluorescein (FITC) excitation/emission settings
• Format allows for continuous measurement of lysozyme activity

The assay utilizes an innovative approach to measure lysozyme activity. Micrococcus lysodeikticus cell walls are used that have been extensively labeled with fluorescein causing quench of the fluorescence signal. Active lysozyme enzyme hydrolyzes the b-(1-4)-glucosidic linkages between the N-acetylmuramic acid and N-acetyl-D-glucosamine residues in the mucopolysaccharide cell wall, relieving the quenching and yielding a dramatic increase in fluorescence that is proportional to lysozyme activity.

사양

검출 방법Fluorescence Intensity

용도(애플리케이션)Lysozyme Assay

용도 (장비)Fluorescence Microplate Reader

제품 유형Lysozyme Assay

제품라인EnzChek™

수량400 assays

배송 조건Room Temperature

기질 특성Lipid-Based Substrate

기질 유형Lysozyme Substrate

타겟 효소Lysozyme

Unit SizeEach