Thermo Fisher Scientific TNF alpha Monoclonal Antibody (4F4)
돼지 TNF alpha를 인식하는 Mouse monoclonal antibody (clone 4F4). Western blot, ELISA, IHC 등 다양한 응용 가능. 인간 및 마우스 TNF와 교차 반응 없음. 단백질 A 정제, 무보존제 액상 형태, -20°C 보관.
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific TNF alpha Monoclonal Antibody (4F4)
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.02 µg/mL | - |
| Immunohistochemistry (IHC) | - | 4 publications |
| Immunocytochemistry (ICC/IF) | - | 1 publication |
| Flow Cytometry (Flow) | - | 2 publications |
| ELISA | 0.1–0.25 µg/mL (coating) | 3 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Pig |
| Published Species | Pig, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 4F4 |
| Immunogen | Recombinant porcine TNF alpha (Baculovirus-derived) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_223611 |
Product Specific Information
MP390 detects natural and recombinant TNF alpha, with a predicted molecular weight of approximately 25 kDa. This antibody does not cross-react with human or mouse TNFα, mouse TNFβ, or porcine TNFβ.
This is a carrier-free, low-endotoxin formulation (<1.0 EU/mg).
ELISA Application:
Can be used as a coating antibody in a sandwich ELISA (100 µg/well) with biotin-labeled TNFα monoclonal antibody (Product # M302B, 0.1–0.65 µg/mL).
Western Blot Application:
Successfully used with 0.02 µg/mL Biotin-conjugated Goat anti-Mouse IgG (Product # 31800), 0.05 µg/mL HRP-conjugated Streptavidin (Product # N100), and recombinant Porcine TNFα (Product # RPTNFAI). Visualization performed using SuperSignal™ West Pico Chemiluminescent Substrate (Product # 34080).
Endotoxin level tested by LAL assay and ≤10 EU/mg.
Target Information
TNF alpha is a multifunctional proinflammatory cytokine belonging to the TNF superfamily. It is mainly secreted by macrophages and binds to receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha regulates immune responses, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation.
It exists as multimeric complexes (dimers, trimers, or pentamers) and appears as a single 17 kDa band on SDS-PAGE under non-reducing conditions. Studies indicate TNF alpha’s role in immune defense, tumor necrosis, and neuroprotection. It has been implicated in autoimmune diseases, insulin resistance, and cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.