Thermo Fisher Scientific E. coli (strain K12) H-NS Polyclonal Antibody
E. coli K12 유래 H-NS 단백질을 인식하는 Rabbit Polyclonal 항체. Western blot에 최적화되어 있으며, Protein G로 정제된 고순도 항체. PBS/glycerol buffer에 보관되며 -20°C 이하에서 안정적. 유전자 전사 억제 및 DNA 결합 연구에 적합.
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Applications
- Western Blot (WB): 1:2,000–1:10,000
- Tested in direct-ELISA
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bacteria |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant E. coli DNA-binding protein H-NS (39–137 aa) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2.615 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS (pH 7.4) with 50% glycerol |
| Contains | 0.03% ProClin 300 |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Wet ice |
| RRID | AB_2902250 |
Product Specific Information
This antibody has been tested in direct-ELISA.
Target Information
H-NS is a DNA-binding protein involved in transcriptional repression (silencing) and bacterial chromosome organization. It binds tightly to AT-rich dsDNA, inhibiting transcription and forming nucleoid clusters that compact DNA. H-NS regulates horizontally transferred genes, controls thermal responses such as pili and curli fimbriae formation, and represses CRISPR-cas promoters. Its DNA-binding cooperativity decreases with temperature, and it can influence transcriptional elongation and translational efficiency of mRNA with weak Shine-Dalgarno sequences.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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