Thermo Fisher Scientific Phospho-ELk1 (Ser389) Polyclonal Antibody
ELK1 단백질의 Ser389 인산화 부위를 특이적으로 인식하는 Rabbit Polyclonal 항체입니다. Western blot, IHC, ICC/IF, ELISA 등 다양한 응용에 적합하며 Human, Mouse, Rat 시료에 반응합니다. Protein A 정제, 액상 형태로 -20°C 보관합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | |
| Immunohistochemistry (Paraffin) (IHC-P) | Assay-dependent | |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 | |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 | |
| Flow Cytometry (Flow) | Assay-dependent | |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic phosphopeptide derived from human ELK1 around the phosphorylation site of Ser389 [PR(p-S)PA]. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
ELK1 is a component of the ternary complex that binds the serum response element (SRE) and mediates gene activity in response to serum and growth factors. It is phosphorylated by MAP kinase pathways at a cluster of serine/threonine motifs at its C-terminus. Phosphorylation at these sites, particularly Ser383, is critical for transcriptional activation by ELK1. ELK1 is a nuclear target for the ras-raf-MAPK signaling cascade, and alternatively spliced transcript variants encoding the same protein have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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