Thermo Fisher Scientific Ly-6A/E (Sca-1) Monoclonal Antibody (D7), Super Bright 645, eBioscience
마우스 Sca-1(Ly-6A/E) 단백질을 인식하는 D7 클론의 Super Bright 645 결합 단클론 항체. 유세포 분석(Flow Cytometry)에 최적화되어 있으며, 405 nm 레이저로 여기되어 645 nm에서 방출. 줄기세포 및 면역세포 표지 연구용.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.5 µg/test | View 38 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Not Applicable |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Super Bright™ 645, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | D7 |
| Conjugate | Super Bright™ 645 |
| Excitation / Emission Max | 414 / 645 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2717113 |
Additional Formats Available
- Unconjugated
- Alexa Fluor 700
- APC
- Biotin
- Brilliant UV 395 / 661 / 737 / 805
- Brilliant Violet 421 / 480 / 605 / 711 / 786
- FITC
- PE
- PE-Cyanine5 / PE-Cyanine7
- PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright 436 / 600
- Request custom conjugation
Product Specific Information
Description:
The D7 monoclonal antibody reacts with mouse Sca-1, an 18 kDa member of the Ly-6 family of GPI-linked surface proteins. D7 recognizes both Ly-6E.1 and Ly-6A.2 molecules expressed by mouse hematopoietic stem cells, myeloid populations, and peripheral T and B cells. Sca-1 expression levels vary by strain and activation state, serving as a key marker for mouse hematopoietic progenitor/stem cell subsets.
Applications Reported:
Reported for use in flow cytometric analysis.
Applications Tested:
Tested by flow cytometric analysis of mouse splenocytes. Recommended ≤0.5 µg per test (100 µL final volume). Optimal dilution should be determined empirically.
Fluorochrome Information:
Super Bright 645 is a tandem dye excited by the violet laser (405 nm) and emits at 645 nm. Use a 660/20 bandpass filter. Ensure the instrument can detect this fluorochrome.
Staining Recommendations:
When using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.
Light Sensitivity:
Sensitive to photo-induced oxidation. Protect from light.
Fixation:
Samples can be stored up to 3 days at 4°C in the dark using:
- IC Fixation Buffer (Product # 00-8222)
- 1-step Fix/Lyse Solution (Product # 00-5333)
Excitation / Emission / Laser:
405 nm / 645 nm / Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
Stem Cell Antigen-1 (Sca-1):
Sca-1 is part of the Ly-6 family and is expressed on multipotent hematopoietic stem cells (HSC). It is widely used as a marker for murine HSC in combination with lineage depletion antibodies. Expression is found in fetal liver, adult bone marrow, peripheral blood, and spleen. Sca-1 may play a role in B and T cell activation regulation.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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