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Thermo Fisher Scientific Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 546
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Thermo Fisher Scientific Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 546

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Goat anti-Mouse IgG (H+L) cross-adsorbed secondary antibody conjugated with Alexa Fluor 546. 높은 특이성과 낮은 배경 신호를 제공하며 ICC/IF 및 Flow Cytometry에 적합. 인간 IgG 및 혈청에 대한 교차흡착으로 신뢰도 향상. 561/572 nm 발광 특성.

카탈로그번호
A11003
판매단위
pk
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마지막 업데이트 2025. 08. 05. 오전 06:18
Thermo Fisher Scientific A11003 Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 546 1 mg pk판매 단위 pk ·
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Thermo Fisher Scientific · Thermo Fisher Scientific Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 546

Applications and Tested Dilution

Application Tested Dilution
Immunocytochemistry (ICC/IF) 4 µg/mL
Flow Cytometry (Flow) 1–10 µg/mL

Product Specifications

Property Description
Species Reactivity Mouse
Host / Isotype Goat / IgG
Class Polyclonal
Type Secondary Antibody
Immunogen Gamma Immunoglobins Heavy and Light chains
Conjugate Alexa Fluor™ 546
Excitation / Emission Max 561 / 572 nm
Form Liquid
Concentration 2 mg/mL
Purification Purified
Storage Buffer PBS, pH 7.5
Contains 5 mM sodium azide
Storage Conditions 4°C, store in dark
Shipping Conditions Ambient
RRID AB_2534071
Target IgG
Cross Adsorption Against human IgG and human serum prior to conjugation
Antibody Form Whole Antibody

Product Specific Information

To minimize cross-reactivity, these goat anti-mouse IgG (H+L) whole secondary antibodies have been affinity purified and cross-adsorbed against human IgG and human serum prior to conjugation. This purification step increases specificity, resulting in higher sensitivity and reduced background staining.

Alexa Fluor 546 dye is a bright, orange-fluorescent dye ideally suited to the 546 nm laser line. It provides stable signal generation and high photostability, allowing detection of low-abundance biological structures with great sensitivity. Each conjugate typically contains 2–8 fluorophore molecules per IgG molecule.

Before use, centrifuge the conjugate solution briefly and use the supernatant to reduce nonspecific staining. Recommended final concentration for fluorophore-labeled antibodies is 1–10 µg/mL for most ICC/IF and flow cytometry applications.

Product is shipped at room temperature.

Target Information

Anti-Mouse secondary antibodies are affinity-purified with well-characterized specificity for mouse immunoglobulins. They are useful for detection, sorting, or purification of targets and provide signal amplification through multiple secondary bindings. These antibodies can be further purified or modified to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures.

  • Agarose
  • Alexa Fluor 350, 405, 430, 488, 514, 532, 555, 568, 594, 633, 635, 647, 660, 680, 700, 750, 790
  • AP, APC, APC-Alexa Fluor 750
  • Biotin, Biotin-XX
  • BODIPY FL, Cyanine3, Cyanine5
  • DyLight 350–800 series
  • FITC, HRP, Pacific Blue, PE, PE-Alexa Fluor 610
  • Rhodamine, Rhodamine Red-X, Texas Red-X, TRITC
  • Custom conjugation available

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