
Thermo Fisher Scientific 14-3-3 zeta Polyclonal Antibody
14-3-3 zeta 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. WB, IHC, ICC/IF에 사용 가능. 고순도(>95%) 항원 친화 크로마토그래피 정제. 1 mg/mL 농도의 액상 형태로 제공되며, 연구용으로만 사용.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Rat, Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 20–70 of Human 14-3-3 zeta |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2802903 |
Product Specific Information
Purity is >95% by SDS-PAGE.
Target Information
The 14-3-3 family consists of at least seven highly conserved isoforms expressed in all eukaryotic cells. These proteins act as key regulators of signal transduction by binding to serine-phosphorylated proteins.
Interactions include:
- Cdc25C: regulates cell cycle entry
- Bad: prevents apoptosis
Other binding partners include protein kinase C family members, Cbl, IRS-1, polyoma middle-T antigen, nitrate reductase, S-raf, and IGF-1 receptor.
Detection of 14-3-3 proteins in cerebrospinal fluid aids in differential diagnosis of Creutzfeldt-Jakob disease and other prion diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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