Thermo Fisher Scientific NAPEPLD Monoclonal Antibody (OTI1H4), TrueMAB
인간 NAPEPLD 단백질을 인식하는 Mouse monoclonal antibody. Western blot과 Immunocytochemistry에 최적화. Lyophilized 형태로 제공되며, PBS buffer와 trehalose 포함. 연구용으로 신경세포 내 NAPE-PLD 관련 연구에 적합.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1H4 |
| Immunogen | Full length human recombinant protein of human NAPEPLD produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
N-Acylphosphatidylethanolamine (NAPE)-hydrolyzing phospholipase D (NAPE-PLD) is a membrane-bound enzyme that releases endocannabinoid anandamide and other bioactive N-acylethanolamines from their corresponding NAPEs in animal tissues.
NAPE-PLD is expressed by specific neuronal populations and localized to axonal processes.
NAEs generated by NAPE-PLD may act as anterograde synaptic signaling molecules regulating postsynaptic neuron activity.
Additionally, NAPE-PLD catalyzes the hydrolysis of NAPE to produce OEA (oleoylethanolamide), a lipid amide that reduces food intake in rodents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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