Thermo Fisher Scientific TUBA3E Monoclonal Antibody (OTI2A8), TrueMAB
Human TUBA3E 단백질을 인식하는 Mouse IgG2a 단일클론 항체로 Western blot, ICC/IF, Flow Cytometry에 사용 가능. Lyophilized 형태로 제공되며, PBS 기반 buffer에 trehalose 포함. 연구용으로 세포 내 미세소관 관련 연구에 적합.
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Thermo Fisher Scientific TUBA3E Monoclonal Antibody (OTI2A8), TrueMAB
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2A8 |
| Immunogen | Full length human recombinant protein of human TUBA3E produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).
Target Information
Microtubules are dynamic intracellular polymers composed of alpha/beta-tubulin heterodimers and associated proteins (MAPs). They exhibit polarity with dynamic plus ends and less dynamic minus ends, essential for processes such as mitosis, meiosis, cell shape maintenance, and intracellular transport.
Alpha-tubulin (~50 kDa) exists as soluble alpha/beta-tubulin dimers or as part of microtubule polymers. In humans, six tubulin genes encode different tubulin classes. Microtubule polymerization is reversible, exhibiting dynamic instability where microtubules alternately grow and shorten at their ends.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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