Thermo Fisher Scientific MDM2 Monoclonal Antibody (IF2)

Applications

Tested Application

• Immunohistochemistry (Paraffin) (IHC (P)): 1:50–1:200

Product Specifications

Product Specific Information

This monoclonal antibody reacts specifically with human MDM2 protein.
Overexpression of MDM2 protein has been detected in a variety of human tumors and appears to result from gene amplification, increased transcript level, and enhanced translation.
Reactivity with mouse MDM2 protein has not been observed.

Expected Staining Pattern: Nuclear

Target Information

MDM2 is a ubiquitin ligase for p53 and plays a central role in regulating the stability of p53.
It is located on chromosome 12q and its phosphorylation by Akt at Ser166 and Ser186 enhances its interaction with p300, leading to MDM2-mediated ubiquitination and degradation of p53.
MDM2 negatively regulates p53 function by inhibiting its transactivation role and promoting its degradation.
Overexpression of MDM2 is observed in various human malignancies, including soft tissue carcinomas and breast cancer.
In addition to p53, MDM2 interacts with other proteins such as retinoblastoma 1 and ribosomal protein L5, influencing processes of cell cycle, apoptosis, and tumorigenesis.
More than 40 alternatively spliced transcript variants of MDM2 have been identified in both tumor and normal tissues.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.