Invitrogen™

Jump-In™ Fast Gateway™ System

The Jump-In™ Fast Gateway™ System is intended for rapid engineering of mammalian cell lines through the stable and irreversible integration자세히 알아보기

The Jump-In™ Fast Gateway™ System is intended for rapid engineering of mammalian cell lines through the stable and irreversible integration of your gene of interest (GOI) at specific genomic loci (pseudo attP sites). Following stable integration, only 5-10 clones need to be analyzed to identify clones with high expression levels.
The Jump-In™ Fast Gateway™ System consists of:

• The Jump-In™ Fast Gateway™ Core Kit containing the promoter-less pJTI™ Fast DEST expression vector and a vector for transient expression of the PhiC31 integrase.
• The MultiSite Gateway™ Pro Plus kit for cloning of up to 4 gene fragments into the promoter-less pJTI™ Fast DEST expression vector.

사양

클로닝 방법Gateway™

구성 또는 유도성 시스템Constitutive

배달 유형Transfection

셀 유형Mammalian

프로모터None (Promoterless)

VectorJump-In Vectors, pJTI

발현 시스템Mammalian

용도(애플리케이션)Protein Expression

고처리량 호환성High-throughput Compatible

주요 기능Stable Cell Line Development, Targeted Integration, Clone your own Promoter

반응 수20 reactions

제품라인Jump-In™

제품 유형Cloning Kit

단백질 태그Untagged

수량20 Reactions

선택 제제(진핵)Hygromycin

선택 마커 진핵HygroR

Unit SizeEach