Thermo Fisher Scientific USP13 Monoclonal Antibody (OTI4G11), TrueMAB
USP13 단백질을 인식하는 Mouse monoclonal 항체로 Western blot, IHC(P), IP에 사용 가능. Human 및 Dog 시료에 반응하며, Affinity chromatography로 정제된 액상 제품. PBS/BSA/glycerol buffer에 보관하며 -20°C에서 안정적 유지.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunoprecipitation (IP) | 4 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI4G11 |
| Immunogen | Full-length protein expressed in 293T cells transfected with human USP13 expression vector |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.41 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Deubiquitinase that mediates deubiquitination of target proteins such as BECN1, MITF, SKP2, and USP10, and is involved in processes such as autophagy and endoplasmic reticulum-associated degradation (ERAD).
Component of a regulatory loop controlling autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, stabilizing PIK3C3/VPS34-containing complexes. Also deubiquitinates USP10, a regulator of p53/TP53 stability.
PIK3C3/VPS34 complexes regulate USP13 stability, suggesting a feedback system controlling p53/TP53 via USP10 and USP13.
Recruited by nuclear UFD1 and mediates deubiquitination of SKP2, regulating ERAD. Also acts on UBL4A in the BAG6/BAT3 complex and stabilizes SIAH2 independently of deubiquitinase activity.
Has weak deubiquitinase activity in vitro, preferentially cleaving Lys-63-linked polyubiquitin chains. Unlike USP5, it does not disassemble unanchored polyubiquitin. May cleave ISG15 in vitro (requires further validation).
[Source: UniProt]
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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