ELK Biotechnology PKC ε (phospho Ser729) rabbit pAb

제품명

PKC ε (phospho Ser729) rabbit pAb

대체 명칭

PRKCE; PKCE; Protein kinase C epsilon type; nPKC-epsilon

적용 분야 (Applications)

• WB
• ELISA
• IHC

권장 희석 배수 (Recommended Dilutions)

면역원 (Immunogen)

The antiserum was produced against a synthesized peptide derived from human PKC epsilon around the phosphorylation site of Ser729.
Amino acid range: 688–737

저장 조건 (Storage)

• Host: Rabbit
• Storage temperature: −20 °C
• Stability: 1 year

항체 특성 (Antibody Properties)

세포 내 위치 (Cellular Localization)

Cytoplasm; cytoskeleton; cell membrane; perinuclear region; nucleus.
Translocated to plasma membrane in epithelial cells stimulated by HGF (PubMed:17603037).
Associated with the Golgi at the perinuclear site in pre-passage fibroblasts.
In passaging cells, translocated to the cell periphery.
Translocated to the nucleus in PMA-treated cells.

배경 (Background)

Protein kinase C epsilon (PRKCE) is a member of the PKC family of serine/threonine kinases activated by calcium and diacylglycerol. PKC family members phosphorylate various protein targets and participate in diverse signaling pathways. They also serve as receptors for phorbol esters, a class of tumor promoters.
PRKCE is involved in multiple cellular functions including neuronal channel activation, apoptosis, cardioprotection from ischemia, heat shock response, and insulin exocytosis. Mouse knockout studies suggest its role in lipopolysaccharide (LPS)-mediated signaling in activated macrophages.

종 반응성 (Species Reactivity)

Human; Mouse; Rat