Himedia Dulbecco`s Modified Eagle Medium / Nutrient Mixture F-12 Ham (DMEM / F12, 1:1 mixture) w/ L-Glutamine, 15mM HEPES buffer and Trace elements w/o Sodium bicarbonate

Dulbecco`s Modified Eagle Medium /Nutrient Mixture F12 Ham (DMEM/F12, 1:1mixture) was originally formulated for rat neuroblastoma cells and MDCK cells. The mixture is extremely nutritious and supports growth of a wide variety of cells including certain epithelial, endothelial and granulosa cells. AT139 is DMEM/ Nutrient Mixture F-12 Ham with Lglutamine, 15mM HEPES buffer and trace elements. HEPES, a zwitterionic buffer having a pKa of 7.3 at 37ºC prevents the initial rise in pH that tends to occur at the initiation of a culture and increases the buffering capacity of the medium.