
Thermo Fisher Scientific Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP
Thermo Fisher Scientific의 Goat anti-Mouse IgG (H+L) HRP 항체는 마우스 IgG 검출용 고감도 2차 항체로, Western blot, IHC, IP 등 다양한 응용에 적합합니다. HRP 결합형으로 신호 증폭이 우수하며, 항원 친화 크로마토그래피로 정제된 고품질 제품입니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:100,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
| Immunoprecipitation (IP) | 1:500–1:5,000 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Mouse |
| Host/Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Purified Mouse IgG, whole molecule |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.6, with 15 mg/mL BSA, 50 mM sucrose |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient |
| RRID | AB_10960845 |
| Target | IgG |
| Antibody Form | Whole Antibody |
Additional Formats Available
- Agarose
- Alexa Fluor 488, 555, 647, 680, 790
- Alexa Fluor Plus 405, 488, 647
- AP, bGal, Biotin
- DyLight 350, 405, 488, 550, 594, 633, 650, 680, 800 4X PEG
- FITC, PE, Rhodamine, TRITC
- WesternDot 625
- Custom conjugation available upon request
Product Specific Information
Product #31431 has been successfully used in Western blot, IHC, and IP applications.
This antibody reacts with the heavy chains of mouse IgG and with the light chains common to most mouse immunoglobulins, but does not react against non-immunoglobulin serum proteins. Cross-reactivity may occur with immunoglobulins from other species.
Store product at 4°C until opened.
To extend shelf-life, add an equal volume of glycerol (final concentration ~50%) and store at -20°C.
Reconstitute with 0.2 mL of distilled water (1 mg/mL after restoration).
Target Information
Anti-Mouse secondary antibodies are affinity-purified and specific for mouse immunoglobulins. They are useful for detection, sorting, or purification of target molecules.
Secondary antibodies enhance versatility and sensitivity by enabling multiple detection systems (HRP, AP, fluorescence) and signal amplification.
These antibodies are typically generated by immunizing the host animal with pooled mouse immunoglobulins and purified via immunoaffinity chromatography. They may also be modified through fragmentation or conjugation to produce highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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