Thermo Fisher Scientific COX5B Polyclonal Antibody
Rabbit polyclonal antibody targeting human COX5B, mitochondrial subunit of cytochrome c oxidase. Validated for WB and IP applications. Predicted cross-reactivity with rat, bovine, pig, and orangutan. Provided as liquid form, unconjugated, 1 mg/mL conce...
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Thermo Fisher Scientific COX5B Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 25 to 75 of human Cytochrome c oxidase subunit 5B, mitochondrial |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Based on 100% sequence identity, this antibody is predicted to react with Rat, Bovine, Pig, and Orangutan
Target Information
The cytochrome c oxidase (COX) family of proteins functions as the final electron donor in the respiratory chain, driving a proton gradient across the inner mitochondrial membrane and resulting in water production.
The mammalian COX apoenzyme is a dimer, each monomer containing 13 subunits (mitochondrial and nuclear).
COX5 is a heme A-containing chain that converts one molecule of oxygen and four molecules of hydrogen to two molecules of water.
Two isoforms exist: COX5a (aerobic) and COX5b (hypoxic).
- COX5a transcription increases under high oxygen conditions.
- COX5b transcription increases under low oxygen conditions to maximize COX apoenzyme turnover.
Usage Note
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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