Thermo Fisher Scientific Phospho-VPS34 (Ser244, Ser249) Polyclonal Antibody
PIK3C3(VPS34) 단백질의 Ser244/249 인산화 상태를 특이적으로 검출하는 Rabbit Polyclonal Antibody. Western blot 및 IHC(Paraffin)에 적합하며, 인산화된 형태의 PI3 Kinase Class III만 인식. 연구용으로만 사용 가능.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:2,000
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:50–1:200
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human PIK3C3 (Accession Q8NEB9), corresponding to amino acid residues around phosphorylated Ser249 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2817107 |
Product Specific Information
Antibody detects endogenous levels of PI3 Kinase Class III only when phosphorylated at Ser244/249.
Target Information
PIK3C3 (hVPS34) is the sole class III member of the PI3 Kinase family.
PIK3C3 phosphorylates phosphatidylinositol (PI) to produce phosphatidylinositol 3-phosphate [PI(3)P], which recruits downstream effectors containing FYVE and Phox domains to membranes.
PIK3C3 is involved in coupling nutrient signaling to mTOR and plays a role in autophagy and intracellular trafficking.
It regulates degradative endocytic trafficking and is required for the abscission step in cytokinesis, likely in the context of PI3KC3-C2.
Also involved in the transport of lysosomal enzyme precursors from early to late endosomes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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