Thermo Fisher Scientific Phospho-VPS34 (Ser244, Ser249) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human PIK3C3(Accession Q8NEB9), corresponding to amino acid residues around phosphorylated Ser249. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:VPS34,uniProtId:Q8NEB9-1,ncbiNodeId:9606,antigenRange:249,antigenLength:887,antigenImageFileName:PA5-105679_VPS34_Q8NEB9-1_Rabbit.svg,antigenImageFileNamePDP:PA5-105679_VPS34_Q8NEB9-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

sequential chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2817107

Product Specific Information

Antibody detects endogenous levels of PI3 Kinase Class III only when phosphorylated at Ser244/249.

Target Information

PIK3C3 (hVPS34) is the sole class III member of the PI3 Kinase family. PIK3C3 phosphorylates phosphatidylinositol (PI) to produce phosphatidylinositol 3-phosphate [PI(3)P], which recruits downstream effectors containing FYVE and Phox domains to membranes. PIK3C3 is involved in coupling nutrient signaling to mTOR and also plays a role in autophagy and intracellular trafficking. Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2. Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for transport from early to late endosomes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.