Thermo Fisher Scientific Human sCD42b Recombinant Protein, PeproTech

Applications

Tested Dilution

Publications

Functional Assay (Functional)

Assay-dependent

-

Product Specifications

Species

Human

Expression System

CHO cells

Amino acid sequence

HPICEVSKVA SHLEVNCDKR NLTALPPDLP KDTTILHLSE NLLYTFSLAT LMPYTRLTQL NLDRCELTKL QVDGTLPVLG TLDLSHNQLQ SLPLLGQTLP ALTVLDVSFN RLTSLPLGAL RGLGELQELY LKGNELKTLP PGLLTPTPKL EKLSLANNNL TELPAGLLNG LENLDTLLLQ ENSLYTIPKG FFGSHLLPFA FLHGNPWLCN CEILYFRRWL QDNAENVYVW KQGVDVKAMT SNVASVQCDN SDKFPVYKYP GKGCPTLGDE GDTDLYDYYP EEDTEGDKVR ATRTVVKFPT KAHTTPWGLF YSWSTASLDS QMPSSLHPTQ ESTKEQTTFP PRWTPNFTLH MESITFSKTP KSTTEPTPSP TTSEPVPEPA PNMTTLEPTP SPTTPEPTSE PAPSPTTPEP TSEPAPSPTT PEPTSEPAPS PTTPEPTPIP TIATSPTILV SATSLITPKS TFLTTTKPVS LLESTKKTIP ELDQPPKLHH HHHHHH

Tag

His-tag

Molecular weight

54.6 kDa

Class

Recombinant

Type

Protein

Purity

≥95% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<1 EU/µg

Activity

Determined by its ability to bind recombinant human von Willebrand Factor-A2 in a functional ELISA.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

Recombinant Human sCD42b/GP1Ba is a 54.6 kDa protein containing 496 amino acid residues that correspond to the extracellular portion of CD42b/GP1Ba, plus a C-terminal His-Tag. Due to glycosylation, it migrates at approximately 100-115 kDa by SDS-PAGE analysis under reducing and non-reducing conditions.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

CD42b is a platelet activation marker involved in the process of coagulation as an aggregating factor. It interacts with extracellular matrix as well as with adhesion molecules. CD42b expression and activation seems to be regulated by VEGF and PDGF. CD42b composes together with GPIb beta, GPIX and GPV the GPIb-IX-V receptor complex critical in the process of platelet-rich thrombus formation by tethering the platelet to a thrombogenic surface. CD42b binds to von Willebrand factor (VWF) exposed at a site of vascular injury, as well as to thrombin, coagulation factors XI and XII, high molecular wight kininogen, TSP-1, integrin Mac-1 and P-selectin. The extracellular domain of CD42b by its interactions also contributes to metastasis. Further, CD42b antigen is expressed on platelets and on megakaryocytes in bone marrow. Several polymorphisms and mutations have been described in the CD42b, some of which are the cause of Bernard-Soulier syndromes and platelet-type von Willebrand disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.