
Thermo Fisher Scientific Filaggrin Recombinant Rabbit Monoclonal Antibody (FLG, 1957R)
Recombinant rabbit monoclonal antibody specific to human Filaggrin (FLG, 1957R). Optimized for IHC-P applications. Produced in HEK293 cells and purified via Protein A/G. Supplied in PBS with 0.05% BSA and sodium azide. For research use only.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC-P) | 1–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | FLG, 1957R |
| Immunogen | Recombinant human Filaggrin protein fragment (around aa 198–288) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For immunohistochemistry (PFA fixed): Incubate antibody for 30 minutes at room temperature.
- For formalin-fixed tissues: Heat tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, then cool at room temperature for 20 minutes before staining.
Target Information
Filaggrin is expressed only in well-differentiated keratinized epithelial cells. Its synthesis varies across different types of ichthyosis and may change under viral, premalignant, or malignant conditions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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