Thermo Fisher Scientific ID3 Monoclonal Antibody (OTI7B6), TrueMAB
ID3 단백질을 표적으로 하는 Mouse IgG1 단일클론 항체로, Western Blot, IHC, ICC/IF에 적합. 고순도 친화 크로마토그래피 정제 제품으로, 인간 시료 반응성. 연구용으로 안정적인 액상 형태 제공.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI7B6 |
| Immunogen | Full length human recombinant protein of human ID3 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.75 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Members of the Id family of basic helix-loop-helix (bHLH) proteins include ID1, ID2, ID3, and ID4. They are ubiquitously expressed and dimerize with members of the class A and B HLH proteins. Due to the absence of the basic region, the resulting heterodimers cannot bind DNA. The ID-type proteins thus appear to negatively regulate DNA binding of bHLH proteins.
Since ID1 inhibits DNA binding of E12 and Myo D, it functions to inhibit muscle-specific gene expression. Under conditions that facilitate muscle cell differentiation, ID protein levels decrease, allowing E12 and/or E47 to form heterodimers with Myo D and myogenin, which activate myogenic differentiation. Expression of each ID protein is strongly dependent on growth factor activation, and reduction of ID mRNA levels by antisense oligonucleotides delays cell cycle reentry following growth factor stimulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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