Thermo Fisher Scientific Ki67 Monoclonal Antibody (OTI1F11), TrueMAB
인간 Ki-67 단백질을 인식하는 마우스 모노클로날 항체로, 세포 증식 마커 분석에 적합. Western blot 및 파라핀 조직 면역조직화학(IHC)에 사용 가능. 고순도 친화 크로마토그래피 정제, 1 mg/mL 농도, 안정적 PBS/BSA/glycerol 버퍼 제공.
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Applications
Western Blot (WB)
- Tested Dilution: 1:200–1:1,000
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:100
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1F11 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1160–1493 of human MKI67 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Ki-67 is a nuclear protein expressed during various stages of the cell cycle, particularly in late G1, S, G2, and M phases. It associates with euchromatin at the perichromosomal layer, centromeric heterochromatin, and the nucleolus via its forkhead-associated (FHA) domain. Ki-67 shows cell cycle-dependent localization: perinucleolar in G1, nuclear matrix in G2, and chromosomal during mitosis. It serves as a proliferation marker since it is absent in G0 cells and increases from G1 through M phase. Ki-67 antibodies are used to determine the proliferative fraction in neoplasms, with prognostic value comparable to thymidine-labelling index. Low Ki-67 index correlates with histologically low-grade tumors. It is routinely used as a neuronal marker of cell cycling and proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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