Thermo Fisher Scientific CD13 Monoclonal Antibody (WM-15 (WM15)), PE-Cyanine7, eBioscience

Thermo Fisher Scientific CD13 Monoclonal Antibody (WM-15 (WM15)), PE-Cyanine7, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.5 µg)/test
• View 6 publications

Product Specifications

Additional Formats

• Unconjugated
• APC
• APC-eFluor 780
• FITC
• PE
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

Description:
WM-15 monoclonal antibody reacts with human CD13 (aminopeptidase N), a transmembrane ectoenzyme expressed on monocytes and granulocytes but not lymphocytes. CD13 is found in various tissues including endothelial cells, epithelial cells, fibroblasts, kidney proximal tubules, intestine, and placenta. It functions in peptide degradation, amino acid scavenging, antigen processing, and cell adhesion/migration.

Applications Reported:
This antibody is reported for use in flow cytometric analysis.

Applications Tested:
Pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. Use at 5 µL (0.5 µg) per test (100 µL final volume). Cell number may range from 10⁵ to 10⁸ cells/test.

Light Sensitivity:
Tandem dye is sensitive to photo-induced oxidation. Protect vial and stained samples from light.

Fixation:
Samples may be stored in IC Fixation Buffer (Product #00-822-49) or 1-step Fix/Lyse Solution (Product #00-5333-54) for up to 3 days at 4°C in the dark with minimal impact on brightness and FRET efficiency.

Excitation/Emission:
488–561 nm excitation; 775 nm emission. Compatible with Blue, Green, and Yellow-Green lasers.

Filtration:
0.2 µm post-manufacturing filtered.

Target Information

CD13 (ANPEP):
A 150–170 kDa type II transmembrane zinc-binding ectopeptidase expressed on various cell types. CD13 catalyzes removal of neutral amino acids from peptides, influencing bioactive peptide activation/inactivation. It plays roles in extracellular matrix degradation, antigen processing, signal transduction, inflammatory response, intercellular contact regulation, cell motility, and vascularization. Expressed by granulocytes, monocytes, and myeloid leukemia cells; associated with poor prognosis in carcinomas. Also functions as an aminopeptidase enzyme and alpha receptor for coronavirus.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지