Thermo Fisher Scientific TRIM28 Polyclonal Antibody
TRIM28 단백질을 인식하는 Goat Polyclonal Antibody로 인간 시료에 반응합니다. WB, IHC, ICC, Flow Cytometry 등 다양한 응용에 사용 가능하며, 고순도(Ammonium sulfate precipitation)로 정제되었습니다. 액상 형태로 -20°C에서 보관합니다.
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Thermo Fisher Scientific TRIM28 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–4 µg/mL |
| Immunocytochemistry (ICC/IF) | 10 µg/mL |
| Flow Cytometry (Flow) | 10 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to the C terminus amino acids SSQELSGGPGDGP (aa 823–835) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Ammonium sulfate precipitation |
| Storage Buffer | TBS, pH 7.3, with 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2078879 |
Product Specific Information
This antibody is tested in Peptide ELISA: antibody detection limit dilution 2,000.
Target Information
The KRAB (Kruppel-associated box) domain, approximately 45 amino acids long, acts as a transcriptional repression domain found in numerous transcription factors. Over 220 KRAB-zinc finger protein (KRAB-ZFP) genes have been identified in the human genome. These proteins repress transcription through interactions with KAP-1 (KRAB-associated protein 1), an 835 amino acid polypeptide containing a RING finger, B boxes, and a PHD finger.
KAP-1 forms complexes with KRAB-domain transcription factors to enhance repression efficiency and directly interacts with HP1 (heterochromatin protein 1) and KRAZ1 (Kruppel-associated box-containing zinc finger protein 1). KAP-1 targets KRAZ1 to centromeric heterochromatin foci containing HP1α, regulating transcriptional repression. Mutant KAP-1 proteins that bind KRAB but fail to bind HP1 lead to random KRAZ1 distribution and strong transcriptional activation.
Usage Note
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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