Thermo Fisher Scientific GFER Polyclonal Antibody
Human 및 Rat 반응성을 가지는 Rabbit Polyclonal GFER 항체로, WB, IHC(P), ICC/IF 등 다양한 응용 가능. 항원 친화 크로마토그래피로 정제되었으며 PBS 기반 용액 형태. 연구용으로만 사용 가능.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 | - |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human GFER. Recombinant protein control fragment (Product #RP-98653). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.3 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2641961 |
Product Specific Information
Immunogen sequence:
QQDMAQFIHL FSKFYPCEEC AEDLRKRLCR NHPDTRTRAC FTQWLCHLHN EVNRKLGKPD FDCSKVDERW RDGWKDGSCD
Highest antigen sequence identity to the following orthologs:
- Mouse: 86%
- Rat: 85%
Target Information
Isoform 1: FAD-dependent sulfhydryl oxidase that regenerates the redox-active disulfide bonds in CHCHD4/MIA40, a chaperone essential for disulfide bond formation and protein folding in the mitochondrial intermembrane space.
The reduced form of CHCHD4/MIA40 forms a transient intermolecular disulfide bridge with GFER/ERV1, resulting in regeneration of the essential disulfide bonds in CHCHD4/MIA40, while GFER/ERV1 becomes re-oxidized by donating electrons to cytochrome c or molecular oxygen.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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