Thermo Fisher Scientific beta-3 Tubulin Monoclonal Antibody (2G10)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 5 publications 5 publications

Immunohistochemistry (IHC)

Assay-dependent

View 10 publications 10 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:50-1:200

View 16 publications 16 publications

Flow Cytometry (Flow)

1:50

-

Immunoprecipitation (IP)

5 µg

-

Product Specifications

Species Reactivity

Bovine, Guinea pig, Hamster, Human, Mouse, Pig, Rabbit, Rat

Published species

Amphibian, Human, Mouse, Rabbit, Rat, Zebrafish

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

2G10

Immunogen

A synthetic peptide corresponding to amino acids 436-450 from rat neuronal specific beta-3 tubulin. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Beta III Tubulin,uniProtId:Q4QRB4-1,ncbiNodeId:10114,antigenRange:436-450,antigenLength:450,antigenImageFileName:MA1-118_Beta_III_Tubulin_Q4QRB4-1_House_mouse.svg,antigenImageFileNamePDP:MA1-118_Beta_III_Tubulin_Q4QRB4-1_House_mouse_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS with 1mg/mL BSA, 30% glycerol

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2536829

Product Specific Information

MA1-118 was successfully used to detect E18 Sparague Dawley primary cortical neurons.

MA1-118 can be used for immunofluorescence analysis of beta III tubulin in the ectoderm derived from human embryonic stem cells.

Western blot analysis of MA1-118 detects an ~50 kDa protein in neuronal-type cells. In bovine, a unknown band at ~32 kDa is also detected. MA1-118 shows specificity to beta-3 Tubulin and is non-reactive to lysates from non-neuronal cell types (e.g. HeLa cell lysate).

Target Information

The betaIII-tubulin isoform is present dominantly in cells of neuronal origin and it is one of the earliest markers of neuronal differentiation. It is regarded as a specific probe for the cells of neuronal origin as well as for the tumours originating from these cells. The betaIII-tubulin is most abundant in cells of neuronal origin, but was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.