Thermo Fisher Scientific CD16 Monoclonal Antibody (3G8), NovaFluor Yellow 755, eBioscience

Thermo Fisher Scientific CD16 Monoclonal Antibody (3G8), NovaFluor Yellow 755, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.6 µg)/test

Species Reactivity

Human, Non-human primate

Product Specifications

Product Specific Information

This 3G8 monoclonal antibody reacts with human and non-human primate CD16, also known as the low-affinity Fc gamma RIII. CD16 exists as two isoforms, FcγRIIIA and FcγRIIIB.

• FcγRIIIA is expressed on monocytes, macrophages, and NK cells.
• FcγRIIIB is found on neutrophils as a GPI-anchored form.
• Expression patterns vary across primate species.

Binding of IgG to CD16 triggers signal transduction pathways leading to antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, cytokine release, and antigen presentation.

Cross-blocking studies indicate that 3G8 recognizes the same epitope as CB16, but distinct from B73.1.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and tested by flow cytometry of human peripheral blood cells. Recommended use: 5 µL (0.6 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

Compatibility Note:
NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assays.

CellBlox Plus Blocking Buffer Usage:
Add 5 µL per sample for optimal staining. For single-color controls, use 5 µL per 100 µL cell sample (10³–10⁸ cells).

NovaFluor conjugates utilize Phiton™ technology based on nucleic acid dye structures, engineered for minimal spillover and spread.

Spectral Information:
Excitation: 552 nm
Emission: 690 nm
Laser: 561 nm (Yellow)

Target Information

CD16 encodes a receptor that binds the Fc portion of IgG, mediating immune complex clearance and cellular cytotoxicity.

• Gene: FCGR3A (chromosome 1)
• Expression: NK cells (transmembrane form), neutrophils (PI-anchored form via FCGR3B)
• Clinical relevance: Mutations linked to Immunodeficiency 20, Herpes Zoster, systemic lupus erythematosus, and neonatal neutropenia.
  Alternative splicing produces multiple isoforms.

For Research Use Only. Not for diagnostic procedures or resale without authorization.

제품 이미지