Thermo Scientific™

Pierce™ SAT(PEG)4 (PEGylated N-succinimidyl S-acetylthioacetate)

Thermo Scientific Pierce SAT(PEG)4 is a long-arm (18.2 angstroms), pegylated form of SATA, a sulfhydryl-addition reagent for covalent modification of자세히 알아보기

Thermo Scientific Pierce SAT(PEG)4 is a long-arm (18.2 angstroms), pegylated form of SATA, a sulfhydryl-addition reagent for covalent modification of primary amines to facilitate controlled, heterobifunctional crosslinking.

Features of SAT(PEG)4:

• Thiolate and PEGylate primary amines in one step
• Add sulfhydryl groups for heterobifunctional crosslinking without compromising protein solubility
• NHS-activated for efficient PEGylation of primary amines at pH 7-9; reaction of NHS-ester group results in formation of stable, irreversible amide bonds
• Pure compound with defined structure and molecular weight, ensuring reproducible protein-modification effects
• PEG spacer provides unique advantages, including increased stability, reduced tendency toward aggregation and reduced immunogenicity

SAT(PEG)4 is the abbreviation for N-Succinimidyl S-acetyl(thiotetraethylene glycol), an amine-reactive reagent for PEGylating and adding sulfhydryl groups to proteins and other amine-containing molecules. Like SATA and other S-acetyl (SAT) reagents, SAT(PEG)4 is most frequently used in bioconjugation methods to incorporate sulfhydryl (—SH) groups into proteins as part of a two-step or heterobifunctional crosslinking strategy. The SAT group prevents oxidation of the sulfur atom but is easily removed by treatment with hydroxylamine when the unprotected sulfhydryl group is needed for conjugation. The polyethylene glycol (PEG) spacer arm in SAT(PEG)4 minimizes steric hindrance and provides added solubility to the modified and conjugated molecules.

Specific reactivity to primary amines (e.g., lysine residues in proteins) occurs via the NHS-ester group. Spontaneous reaction in phosphate or other non-amine buffer at pH 7-9 results in formation of stable, covalent, amide bonds. Upon deprotection of the sulfhydryl group with hydroxylamine, molecules that have been modified with SAT(PEG)4 can be reacted with other proteins or surfaces that have been maleimide-activated with heterobifunctional crosslinkers like Sulfo-SMCC or SM(PEG)n Reagents.

Related Products
Pierce™ SATA

사양

화학물질 반응성Amine

라벨 또는 염료Sulfhydryl Group

라벨 유형Conjugating Groups

제품라인Pierce™

교차결합제 유형Heterobifunctional

반응성 부분Active Ester, Succinimidyl Ester, NHS Ester

스페이서Long, PEGylated

형태Liquid

수량100 mg

용해도DMF, DMSO

제품 유형Crosslinker

Unit SizeEach