Invitrogen™

MEGAscript™ RNAi Kit with Manual

The Ambion™ MEGAscript™ RNAi Kit is for the synthesis of large mass amounts of dsRNA for RNAi experiments in nonmammalian자세히 알아보기

The Ambion™ MEGAscript™ RNAi Kit is for the synthesis of large mass amounts of dsRNA for RNAi experiments in nonmammalian systems. The MEGAscript RNAi Kit uses Ambion`s patented high-yield transcription technology and includes reagents for transcription, nuclease digestion, and clean up. The kit includes sufficient reagents for 20 reactions.

Synthesize Large Amounts of dsRNA:
The ability to generate large amounts of clean, double-stranded RNA is essential for the success of RNAi experiments. The MEGAscript RNAi Kit allows RNAi researchers to synthesize 50 to 100 μg or more of double-stranded RNA in a single transcription reaction. The yield is typically 10–50 times the amount of RNA produced by conventional transcription reactions. DNA of either PCR or plasmid origin can be used as a template for the transcription reaction. The sense and antisense strands can be synthesized from a PCR-generated DNA template containing T7 RNA Polymerase promoters on both ends of the template, eliminating the need for a separate annealing step.

사양

제어 유형Transcription Control

샘플 종류Double-Stranded RNA (Long)

반응 수20 reactions

제품라인Ambion™, MEGAscript™

수량20 reactions

배송 조건Wet Ice

Unit SizeEach