Thermo Fisher Scientific Goat anti-Human IgG Fc Recombinant Secondary Antibody, Alexa Fluor 647

Applications

Tested Dilution

Western Blot (WB)

1:1,000

Immunocytochemistry (ICC/IF)

1:500-1:2,000

Flow Cytometry (Flow)

1:1,000

Product Specifications

Species Reactivity

Human

Host/Isotype

Goat / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Secondary Antibody

Clone

3H8L9

Immunogen

Purified Human IgG

Conjugate

Alexa Fluor™ 647 Alexa Fluor™ 647 Alexa Fluor™ 647

View additional formats

• [Alexa Fluor 488 ](/antibody/secondary/product-name/Human IgG Fc/conjugates/Alexa Fluor 488/host/Goat/)
• [Alexa Fluor Plus 488 ](/antibody/secondary/product-name/Human IgG Fc/conjugates/Alexa Fluor Plus 488/host/Goat/)
• [Alexa Fluor Plus 647 ](/antibody/secondary/product-name/Human IgG Fc/conjugates/Alexa Fluor Plus 647/host/Goat/)
• [AP](/antibody/secondary/product-name/Human IgG Fc/conjugates/AP/host/Goat/)
• [Biotin ](/antibody/secondary/product-name/Human IgG Fc/conjugates/Biotin/host/Goat/)
• [FITC](/antibody/secondary/product-name/Human IgG Fc/conjugates/FITC/host/Goat/)
• [HRP](/antibody/secondary/product-name/Human IgG Fc/conjugates/HRP/host/Goat/)
• [PE ](/antibody/secondary/product-name/Human IgG Fc/conjugates/PE/host/Goat/)
• [TRITC](/antibody/secondary/product-name/Human IgG Fc/conjugates/TRITC/host/Goat/)
• Request custom conjugation

Excitation/Emission Max

650/671 nm View spectra

Form

Liquid

Concentration

1.0 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4

Contains

5mM sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Wet ice

RRID

AB_2925774

Target

IgG Fc

Antibody Form

Whole Antibody

Product Specific Information

This Goat anti-Human IgG Fc Recombinant Secondary Antibody specifically detects human IgG Fc with no cross-reactivity to human IgA or IgM or various species immunoglobulins.

The sensitivity and specificity of each lot is confirmed using ELISA.

Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include: better specificity, animal origin-free formulation, and lot-to-lot consistency.

It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Target Information

Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.