Thermo Fisher Scientific ACT1 Monoclonal Antibody (9ACT12), eBioscience

Applications

Tested Dilution

Publications

Western Blot (WB)

1-5 µg/mL

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

-

View 1 publication 1 publication

Flow Cytometry (Flow)

-

View 1 publication 1 publication

Immunoprecipitation (IP)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rat / IgG1

Class

Monoclonal

Type

Antibody

Clone

9ACT12

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1723991

Product Specific Information

Description: The monoclonal antibody 9ACT12 reacts with human Act1 (NF-kappaB activator 1), also known as CIKS (a connection to IkB kinase and stress-activated kinase). Originally cloned for its ability to activate NF-kappaB and its direct interaction with IKKgamma, Act1 has been subsequently shown to play several roles in immunity. In B cells, it is a negative regulator of CD40 and BAFFR signaling affecting reduced survival. This regulation is through direct interaction of Act1 with CD40 or BAFFR and TRAF3. In addition, Act1 is involved in IL-17R signaling. It interacts with IL-17RA through the homotypic SEFIR interaction domain and is critical for downstream NF-kappaB- and C/EBP-mediated inflammatory responses.

Applications Reported: This 9ACT12 antibody has been reported for use in immunoblotting (WB).

Applications Tested: This 9ACT12 antibody has been tested by immunoblot of human peripheral blood cell lysates. This can be used at less than or equal to 1-5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

This gene encodes a protein involved in regulating responses to cytokines by members of the Rel/NF-kappaB transcription factor family. These factors play a central role in innate immunity in response to pathogens, inflammatory signals and stress. This gene product interacts with TRAF proteins (tumor necrosis factor receptor-associated factors) and either I-kappaB kinase or MAP kinase to activate either NF-kappaB or Jun kinase. Two alternative transcripts encoding different proteins have been identified. A third transcript, which does not encode a protein and is transcribed in the opposite orientation, has been identified. Overexpression of this transcript has been shown to reduce expression of at least one of the protein encoding transcripts, suggesting it has a regulatory role in the expression of this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.