Thermo Fisher Scientific ACT1 Monoclonal Antibody (9ACT12), eBioscience

Thermo Fisher Scientific ACT1 Monoclonal Antibody (9ACT12), eBioscience

Applications and Tested Dilution

Product Specifications

Product Specific Information

The monoclonal antibody 9ACT12 reacts with human Act1 (NF-kappaB activator 1), also known as CIKS (connection to IkB kinase and stress-activated kinase). Act1 is involved in NF-κB activation and interacts directly with IKKγ. It plays multiple roles in immunity, acting as a negative regulator of CD40 and BAFFR signaling in B cells, reducing survival through direct interaction with TRAF3.

Additionally, Act1 participates in IL-17R signaling, interacting with IL-17RA via the SEFIR domain, essential for NF-κB and C/EBP-mediated inflammatory responses.

• Applications Reported: Immunoblotting (WB)
• Applications Tested: Human peripheral blood cell lysates (1–5 µg/mL recommended)
• Purity: >90% (SDS-PAGE)
• Aggregation: <10% (HPLC)
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

This gene encodes a protein regulating cytokine responses via the Rel/NF-κB transcription factor family, central to innate immunity against pathogens and stress. The gene product interacts with TRAF proteins, I-kappaB kinase, or MAP kinase to activate NF-κB or Jun kinase pathways.
Three transcript variants have been identified, one of which acts in a regulatory role by reducing expression of protein-coding transcripts.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.