Thermo Fisher Scientific EHD1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.03-0.1 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

3.75 µg/mL

-

Immunocytochemistry (ICC/IF)

10 µg/mL

-

Flow Cytometry (Flow)

10 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide with sequence FSWVSKDARRKKEPC (aa 2-15). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:EHD1,uniProtId:Q9H4M9-1,ncbiNodeId:9606,antigenRange:2-15,antigenLength:534,antigenImageFileName:PA5-142121_EHD1_Q9H4M9-1_Goat.svg,antigenImageFileNamePDP:PA5-142121_EHD1_Q9H4M9-1_Goat_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Ammonium sulfate precipitation

Storage buffer

TBS, pH 7.3, with 0.5% BSA

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2932780

Product Specific Information

This antibody is tested in Peptide ELISA: antibody detection limit dilution 1:32,000.

Target Information

EHD1 acts in early endocytic membrane fusion and membrane trafficking of recycling endosomes. EHD1 also plays a role in myoblast fusion and is recruited to endosomal membranes upon nerve growth factor stimulation, indirectly regulates neurite outgrowth. Further, EHD1 belongs to a highly conserved gene family encoding EPS15 homology (EH) domain-containing proteins. The protein-binding EH domain was first noted in EPS15, a substrate for the epidermal growth factor receptor. The EH domain has been shown to be an important motif in proteins involved in protein-protein interactions and in intracellular sorting. The EHD1 protein is thought to play a role in the endocytosis of IGF1 receptors. Alternatively spliced transcript variants have been found for the EHD1 gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.