Thermo Fisher Scientific HEY1 Polyclonal Antibody
Human HEY1 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB 및 ICC/IF에 최적화되어 있습니다. 고순도 항원 친화 크로마토그래피로 정제되었으며, Notch 신호 경로 연구 및 심혈관 발달 관련 단백질 분석에 적합합니다.
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Applications
Western Blot (WB)
- Tested Dilution: 1:1,000–1:10,000
- Publications: –
Immunocytochemistry (ICC/IF)
- Tested Dilution: 1:100–1:2,000
- Publications: –
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to amino acids 12–304 of Human HEY1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.04 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2548550 |
Product Specific Information
- Recommended positive controls: DDDDK-tagged HEY1-transfected 293T cells
- Predicted reactivity: Mouse (96%), Rat (89%), Xenopus laevis (81%), Dog (94%), Bovine (93%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Hairy/enhancer-of-split related with YRPW motif protein 1 (HEY1) belongs to the HEY family and is a Notch Signaling Pathway protein. It contains one basic helix-loop-helix (bHLH) domain and one Orange domain. HEY1 acts as a transcriptional repressor binding preferentially to the canonical E-box sequence (5’-CACGTG-3’). It represses transcription by cardiac activators GATA4 and GATA6 and is expressed during nervous system development. HEY1 may be required for cardiovascular development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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