Thermo Fisher Scientific MHC Class I (H-2Db) Monoclonal Antibody (28-14-8), NovaFluor Blue 690, eBioscience

Thermo Fisher Scientific MHC Class I (H-2Db) Monoclonal Antibody (28-14-8), NovaFluor Blue 690, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 1.0 µg/test

Product Specifications

Additional Formats Available

• Unconjugated
• APC
• Biotin
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 510, Blue 585, Blue 610-70S, Blue 660-120S
• NovaFluor Red 710, Red 755
• NovaFluor Yellow 570, Yellow 660, Yellow 730, Yellow 755
• PE, PE-Cyanine7, PerCP-eFluor 710, Super Bright 436
• Custom conjugation available

Product Specific Information

The 28-14-8 monoclonal antibody reacts with mouse MHC class I, H-2D^b, and cross-reacts with H-2L^d, H-2D^q, and/or H-2L^q. Binding to the alpha 3 domain of H-2L^d is independent of beta2-microglobulin.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse splenocytes at ≤1 µg/test (100 µL final volume, 10^5–10^8 cells/test). Titrate for optimal performance.

Important Notes:

• NovaFluor dyes are incompatible with DNA intercalating viability dyes (e.g., PI, 7-AAD, DAPI).
• Recommended viability stains: Invitrogen LIVE/DEAD Fixable Dead Cell stains.
• Use CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) for optimal staining. Add 5 µL per sample for antibody cocktails or single-color controls.
• NovaFluor conjugates utilize Phiton™ technology for engineered fluorescent signatures with minimal spillover and spread.

Excitation: 494 nm
Emission: 585 nm
Laser: 488 nm (Blue)

Target Information

MHC class I alloantigen involved in antigen presentation to CD3/TCR and CD8-expressing T cells.
HLA and MHC antibodies are essential in Immunopeptidomics for neoantigen identification via LC-MS/MS.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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