Thermo Fisher Scientific Nephrin Polyclonal Antibody
Rabbit polyclonal antibody against human Nephrin. Validated for WB and ICC/IF. High specificity and affinity purified. Supplied as liquid in PBS with glycerol and BSA. Suitable for kidney tissue and cell line studies.
- 카탈로그번호
- PA5109310
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Nephrin Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein within Human Nephrin aa 1,042–1,241 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol, 0.2% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, avoid freeze/thaw cycles, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2854721 |
Product Specific Information
Positive Control: Human kidney tissue, 293T, PANC-1, PC-12.
Target Information
Primary steroid-resistant nephrotic syndrome (SRNS) is characterized by childhood onset of proteinuria and progression to end-stage renal disease. Kidney podocytes and their slit diaphragms form the final barrier to urinary protein loss.
Congenital nephrotic syndrome (CNS) is caused by mutations in NPHS1 (nephrin) or NPHS2.
Nephrin, a recently identified protein, is a major component of the slit diaphragm, especially in podocyte foot processes. It functions as a cell adhesion molecule, crucial for maintaining the glomerular filtration barrier.
Mutations in the nephrin gene are linked to congenital nephrosis. Nephrin, along with Podocin and α-Actinin-4, plays a key role in podocyte structure and function. It is also implicated in anti-apoptotic activity and VEGF signaling pathways, contributing to glomerular integrity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.