Thermo Fisher Scientific Bora Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 260-559 of human BORA (NP_079084.4).

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.97 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.01% thimerosal

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2805596

Product Specific Information

Immunogen sequence: KYSLGSITSP SPISSPTFSP IEFQIGETPL SEQRKFTVHS PDASSGTNSN GITNPCIRSP YIDGCSPIKN WSPMRLQMYS GGTQYRTSVI QIPFTLETQG EDEEDKENIP STDVSSPAMD AAGIHLRQFS NEASTHGTHL VVTAMSVTQN QSSASEKELA LLQDVEREKD NNTVDMVDPI EIADETTWIK EPVDNGSLPM TDFVSGIAFS IENSHMCMSP LAESSVIPCE SSNIQMDSGY NTQNCGSNIM DTVGAESYCK ESDAQTCEVE SKSQAFNMKQ DHTTQRCWMK TASPFQCSSP; Positive Samples: HT-29, HeLa

Target Information

Bora (Protein aurora borealis) is a key activator of Aurora Related Protein Kinase A (ARK-1), which is a centrosome-associated serine/threonine kinase that regulates centrosome maturation, bipolar spindle assembly and chromosome segregation during mitosis. Bora is localized to the nucleus until mitosis is initiated, then translocates to the cytoplasm in a Cdc2 dependent manner. Activation of Cdc2 initiates the release of Bora into the cytoplasm where it can bind and activate ARK-1. PLK1 (polo-like kinase-1) interacts with Bora to control the accessibility of its activation loop for phosphorylation and activation by ARK-1. Bora and ARK-1 cooperatively activate PLK1 and control mitotic entry. Bora mutants result in multipolar spindles in mitosis identical to those observed when ARK-1 function is blocked. Thus, the ARK1-Bora-PLK1 regulatory circuit in mammalian cells elucidates a key mechanism in cell cycle regulation. At least three isoforms of Bora are known to exist.

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For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.